12-ID-B routinely conducts simultaneous SAXS/WAXS measurements on biological macromolecules (protein, nucleic acids, complexes, etc) and other aqueous solution samples.
Batch mode
- Room temperature autosampler
- Variable temperature autosampler
FPLC (size exclusion SAXS)
- Available SEC Columns:
- Superdex 75 Increase 5/150 GL, MW: 3-70KDa; bed volume: 3ml; typical running time: 20-30min
- Superdex 75 Increase 10/300 GL, MW: 3-70KDa; bed volume: ~24ml; typical running time: 1-2hrs
- Superdex 200 Increase 5/150 GL, MW: 10-600KDa; bed volume: 3ml; typical running time: 20-30min
- Superdex 200 Increase 10/300 GL, MW: 10-600KDa; bed volume: ~24ml; typical running time: 1-2hrs
- Superose 6 Increase 10/300 GL, MW: 5-5000KDa; bed volume: ~24ml; typical running time: 1-2hrs
Stop flow (time resolved SAXS)
Batch Mode Requirements
Samples must be pure and stable. The volume of each sample / buffer is >=100 uL (140-160uL is easier for operation and recommended if having sufficient materials). To correct possible structure factor (influence of inter-particle interaction among molecules under study), three or more concentrations are recommended for each type sample/molecule, e.g., 1, 2, 4 mg/ml. Besides samples, matching buffers are also required for background measurements. To reduce the influence of possible beam drifting, measurements are done in the following order: one buffer, 3-4 samples, one buffer, 3-4 samples, ...
Autosampler Requirements
Shipping Address
- Name of Beamline Contact c/o your name
- Building 433-E030 (Sector 12)
- 9700 South Cass Ave
- Lemont, IL 60439
Instructions
- There is no package delivery on Saturdays, Sundays, and holidays; please plan in advance.
- Indicate on the outside of the carton storage conditions: frozen or 4 degrees C
- Batch mode / Eppendorf tubes
- 96 well plates
User Registration and Sample Holder Request
- link
Holder request for 96 well plate ONLY
- link
Holder request for Eppendorf tube
- link
Manual for Autosampler
- link
BioSAXS workshop
- link
BioSAXS analysis
- link